We are a group of Promega scientists and science writers who are blogging about science (and some other things) that we think will be interesting and of value to other scientists.
Promega Connections's Latest Posts
You know the scene: you are working on a key experiment on a weekend or after hours–finishing up before an upcoming meeting or before a grant update or proposal submission. You go to the laboratory freezer for that enzyme or protease, or worse to the shelf for the nucleic acid extraction kit for all those samples […]
Mitochondria, often thought of as powerhouses of the cell, are fascinating eukaryotic organelles with a double-layered membrane and their own genome. Mitochondrial DNA (Mt DNA) is typically about 16570 bases, circular, highly compact, haploid and contains 37 genes, all of which are essential for normal mitochondrial function. Thirteen of these genes provide instructions for making […]
Q: What is the easiest way to clone PCR Products? A: The simplest way to clone PCR Products is to amplify the product using thermostable polymerases such as Taq, Tfl or Tth polymerase. These polymerases add a single deoxyadenosine to the 3´-end of the amplified products (3´-end overhang), and can be cloned directly into a […]
Your Promega Connections bloggers thought we would inject a little levity into the day. So we are sharing this Ed Himelblau cartoon limerick. Are any of our readers poets? Do you have a couplet, haiku or science-related limerick you can share with us?
Understanding the course of a pathogenic infection involves not only understanding what ultimately kills the host or how the bacterium or virus enters the body but also how it establishes itself in the host organism. What is the receptor that allows a virus to enter the cell? Which cells does a bacterium first target or […]
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