We are a group of Promega scientists and science writers who are blogging about science (and some other things) that we think will be interesting and of value to other scientists.
Promega Connections's Latest Posts
Reporter assays using a single reporter, be it from a stable cell line or transient transfection, can benefit from normalization. Obviously, we are not talking about adding a second control reporter but normalizing to the number of live or dead cells in the well. Two cell health assays, CellTiter-Fluor™ Cell Viability Assay and CellTox™ Green […]
2015 is the International Year of Light, and activities around the globe are planned to celebrate light in nature, the scientists who have helped us understand the nature of light and the engineers who have developed countless tools and technologies harnessing the power of light. At Promega, our favorite kind of light in nature is […]
Consider the microRNA. At only about 21–26 nucleotides in length, microRNAs (miRNAs) are short, but don’t dismiss miRNAs as too short to accomplish much of anything. miRNAs are a multifunctional workhorse that play a key role in a number of genetic regulatory mechanisms throughout the plant and animal kingdoms and even in certain viruses. Scientists […]
Many studies, from reporter assays to protein localization to BRET and FRET, require successful transfection first. Yet, transfection can be tricky and difficult. There are many considerations when planning transfection of your cells including reagent selection, stable or transient experiment, type of molecule and endpoint assay used. Here we discuss these considerations to help you […]
When I was in the lab, we usually started with an elaborate system of borrowed hairdryers and old chemistry ring stands. What is your preferred method of attacking a frost-full freezer?
Log in to leave a comment