We are a group of Promega scientists and science writers who are blogging about science (and some other things) that we think will be interesting and of value to other scientists.
Promega Connections's Latest Posts
Identification of a crime perpetrator on the basis of DNA fingerprinting is not as easy as some of the CSI shows on television make it out to be. A sample such as blood stain, touch sample or body fluid retrieved at a crime scene is often a challenge for DNA analysts. In many instances, the […]
It is nearly one month past the summer solstice and here in southern Wisconsin, the glow of fireflies at dusk and into darkness is a summertime pleasure not to be missed. Fireflies employ the substrate luciferin and the enzyme luciferase, to produce their bioluminescence. The flash of a firefly lantern is designed to attract a […]
If battlegrounds could speak they would have many stories to tell. In some cases the microbes found in those soils have lived on to separate fact from fiction. One such story has its origins in the Battle of Shiloh, which went down in history as one of the bloodiest battles fought during the American Civil […]
Many different polypeptide fusion partners or affinity tags have been developed to facilitate purification of target proteins. The most commonly used tag for the purification and detection of recombinant expressed proteins is the His tag. Cloning vectors designed to generate His-tagged proteins contain 5–10 histidine residues at either the C- or N terminus of the expressed […]
In May of 1986, a woman in Orange County, Florida, was surprised by a man who entered her apartment and raped her at knifepoint. Despite the fact that she got a glimpse of his face, the chances of identifying and convicting her rapist were slim. Although law enforcement officers did their best to identify the […]
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