We are a group of Promega scientists and science writers who are blogging about science (and some other things) that we think will be interesting and of value to other scientists.
Promega Connections's Latest Posts
My one attempt at working with plant DNA when I was at the lab bench was trying to create a shotgun library from a rice BAC. Never have I needed to isolate nucleic acid from the source material, but based on my conversations with plant scientists, it can be problematic endeavor between the tough tissue […]
Before you begin your subcloning, you need to know: The restriction enzyme (RE) sites available for subcloning in your parent vector multiple cloning region (or in the insert if you need to digest the insert); the RE sites available in the destination vector multiple cloning region (MCR); and if these same sites also occur in […]
Transcriptional activator-like effector nucleases (TALENs) have rapidly become a technique of choice for precision genome engineering. TALENs are custom-designed nucleases that consist of a modular DNA-binding domain fused to a monomeric, C-terminal FokI nuclease domain (1). TALENs work in pairs and are designed to recognize and bind to tandem-oriented sequences in genomic DNA, separated by […]
Last week (September 29–October 2), I was one of almost 1,000 people who attended the 25th International Symposium on Human Identification (ISHI25) in Phoenix, Arizona. This scientific meeting brings together DNA analysts from forensic and paternity labs, research scientists and others with an interest in DNA-based identification to learn about new technologies, policy and process […]
Once the domain of analytical chemistry labs, mass spectrometry instruments are now used in basic life science research, drug discovery research, environmental and industrial laboratories, and in many other biological laboratories. These instruments, which are used to perform critical analyses, need to be monitored for performance and quality. How do you determine the system suitability […]
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